Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs

A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a member of the G-protein-coupled receptor family, is reported. The peptide-resin conjugates were subsequently shown to interact specifically with a fraction of proteins present in cellular lysates.

Graphical abstractThe synthesis of affinity supports designed to identify GPCR partners is reported.Figure optionsDownload full-size imageDownload as PowerPoint slide