| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1367739 | Bioorganic & Medicinal Chemistry Letters | 2005 | 4 Pages |
Abstract
The DNA polymerase reaction by Klenow fragment (KF) was efficiently regulated with UV light using a 25-mer caged fluorescent oligodeoxynucleotide (CFO) as the template. The CFO was functionalized with a fluorescein reporter (Fl) and photocleavable DABSYL quencher moiety (Dab). With Fl and Dab at adjacent cytidines in the middle at the template, KF was blocked from extending a complementary 12-mer primer. Upon UV photolysis of the DABSYL blocking group under aerobic conditions, fluorescein emission was restored and 50% of the primers were fully extended by KF.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
XinJing Tang, Julia L. Richards, Adam E. Peritz, Ivan J. Dmochowski,