Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1368831 | Bioorganic & Medicinal Chemistry Letters | 2014 | 4 Pages |
Abstract
For the development of the excimer probe responsible for DNA target, the deoxyuridine phosphoramidite derivative bearing the silylated pyrene attached at the C-5 position was prepared and incorporated into oligonucleotides. The modified oligonucleotides showed the excimer emission in the absence of the target DNA, on the other hands, the excimer emission was quenched in the presence of the target DNA. For the utilization of the fluorescence behavior, the novel molecular beacon probe containing the silylated pyrene-modified nucleoside at the stem region was designed and the fluorescence property of the probe found to show the responsibility for DNA target.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Tomohisa Moriguchi, Mayumi Ichimura, Mitsuhisa Kato, Kenya Suzuki, Yuki Takahashi, Kazuo Shinozuka,