Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1369197 | Bioorganic & Medicinal Chemistry Letters | 2013 | 4 Pages |
Abstract
To increase base recognition capability and sensitivity, we propose the separation of a commonly used single-probe system for oligonucleotide analysis into a set of three probes: a fluorophore-labeled probe, a promoter probe, and a short probe. In this study, we found that the probes of only 4 nt in length can selectively bind the corresponding gap site on complexes consisting of the target, fluorophore-labeled probe, and promoter probe, exhibiting a more than 14-fold difference in ligation between the matched and mismatched sequences. Moreover, we demonstrated that the immobilized short probes accurately recognized the sequences of the gap sites.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Ken Yamada, Akihiro Ohkubo, Yousuke Esaka, Takashi Kanamori, Yoshiaki Masaki, Kohji Seio, Mitsuo Sekine,