Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1369348 | Bioorganic & Medicinal Chemistry Letters | 2013 | 4 Pages |
Abstract
At pH from 5.5 to 7.6, absorptivity of 4-nitro-1-naphthol at 450 nm is over 2.1-fold of that of para-nitrophenol at 405 nm and over 9.6-fold of that of ortho-nitrophenol at 415 nm. On 4-nitro-1-naphthyl-β-d-galactopyranoside at pH 7.4, catalytic efficiency of Escherichia coli β-d-galactosidase is 3-fold of that on para-nitrophenyl-β-d-galactopyranoside and about 40% of that on ortho-nitrophenyl-β-d-galactopyranoside, and produces a lower quantification limit of penicillin G by enzyme-linked-immunoabsorbent-assay. Hence, 4-nitro-1-naphthol is favorable to prepare chromogenic substrates of hydrolytic enzymes of neutral or slightly acidic optimum pH.
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Related Topics
Physical Sciences and Engineering
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Organic Chemistry
Authors
Jizheng Dang, Hongbo Liu, Xiaolan Yang, Yi Zhang, Yanling Xie, Yuanli Li, Jun Pu, Juan Liao, Yonghua Yuan, Fei Liao,