Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1369398 | Bioorganic & Medicinal Chemistry Letters | 2013 | 7 Pages |
We synthesized various C8-naphthylethynylated 2′-deoxyadenosine derivatives and investigated their photophysical properties. Among them, cyano- and N,N-dimethylamino-substituted 8-naphthylethynylated 2′-deoxyadenosine derivatives (cnA and dnA) showed strong fluorescence with high quantum yields and a remarkable solvatofuorochromicity. In particular, fluorescence of N,N-dimethylamino-substituted 2,6dnA was not quenched by neighboring guanines (Gs) when incorporated in DNA duplexes, in contrast to cnA. We developed a new fluorescent probe containing 2,6dnA that can be used for the detection of target DNA via a bulge formation regardless of the neighboring sequences.
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