Synthesis and polymerase incorporation of β,γ-modified α-l-threofuranosyl thymine triphosphate mimics

Three β,γ-modified α-l-threofuranosyl nucleoside triphosphates were synthesized. The β,γ-modified tTTPs undergo a single incorporation event with HIV RT but undergo multiple incorporations to form full-length product with engineered thermophilic polymerases.

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