| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1370544 | Bioorganic & Medicinal Chemistry Letters | 2015 | 6 Pages |
Abstract
A spermine-conjugated ethyl phosphotriester oligonucleotide was obtained by solid-phase synthesis based on phosphoramidite chemistry. The ethyl phosphotriester linkage was robust to exonuclease digestion and stable in fetal bovine serum. Cell membrane permeability of the spermine-conjugated ethyl phosphotriester oligonucleotide was studied by fluorescence experiments. The effective cell penetrating potency of the spermine-conjugated ethyl phosphotriester oligonucleotide was determined by confocal laser scanning microscopy and measurement of intracellular fluorescence intensity.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Junsuke Hayashi, Tomoko Hamada, Ikumi Sasaki, Osamu Nakagawa, Shun-ichi Wada, Hidehito Urata,