Synthesis of d-labeled and unlabeled benzoyloxysuccinimides and application to quantitative analysis of peptides and a protein by isotope differential mass spectrometry

Benzoyloxysuccinimide and its d5-labeled version, which react with amino groups in the N-termini and lysine side chains in proteins, were synthesized and applied to quantitative analysis of peptides and a commercially available protein in combination with a MALDI mass spectrometer.

Graphical abstractD5-labeled and unlabeled benzoyloxysuccinimides that covalently react with amino groups have been synthesized and applied to quantitative analysis of peptides and a protein.Figure optionsDownload full-size imageDownload as PowerPoint slide