Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1370950 | Bioorganic & Medicinal Chemistry Letters | 2011 | 4 Pages |
Abstract
CEs are important enzymes that catalyze the hydrolysis of prodrugs. In this Letter, we present a new mechanistic ER-specific fluorescent probe 1 based on CE activity. Permeation of 1 into cells and subsequent hydrolytic activation by CEs causes spontaneously quinone methide cleavage, resulting in bright red fluorescence in ER with high specificity. Probe 1 was developed for CE activity imaging and inhibitor screening at the cellular level.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Wataru Hakamata, Aki Machida, Tadatake Oku, Toshiyuki Nishio,