| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1371018 | Bioorganic & Medicinal Chemistry Letters | 2015 | 4 Pages |
Abstract
A fluorogenic assay for SIRT5 has been developed to screen their small molecule modulators based on the recent discovery that SIRT5 is a demalonylase and desuccinylase. However, this assay uses a fluorogenic peptide containing 7-amino-4-methylcoumarin (AMC), which becomes the cause of false positive hits from the screening. To overcome this, we have developed an alternative method called a FRET-based assay, which will be reliable and useful for screening SIRT5 modulators in a high-throughput format since no AMC group present in this assay.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Yan Li, Wenfei Huang, Ling You, Ting Xie, Bin He,