Artificial leucine rich repeats as new scaffolds for protein design

The leucine rich repeat (LRR) motif that participates in many biomolecular recognition events in cells was suggested as a general scaffold for producing artificial receptors. We describe here the design and first total chemical synthesis of small LRR proteins, and their structural analysis. When evaluating the tertiary structure as a function of different number of repeating units (1–3), we were able to find that the 3-repeats sequence, containing 90 amino acids, folds into the expected structure.

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