Synthesis of novel oxime-containing pyrazole derivatives and discovery of regulators for apoptosis and autophagy in A549 lung cancer cells

A series of novel oxime-containing pyrazole derivatives were synthesized by the reaction of ethyl 3-phenyl-1H-pyrazole-5-carboxylate derivatives and 2-bromo-1-phenylethanone followed by the reaction with hydroxylamine hydrochloride. The structures were determined by IR, 1H NMR, HRMS, and X-ray analysis. A dose- and time-dependent inhibition of proliferation was observed in A549 lung cancer cell after compound treatment. Inhibition of growth was mainly attributed to the autophagy induction.

Graphical abstractA series of novel oxime-containing pyrazole derivatives were synthesized and characterized by IR, 1H NMR, HRMS spectroscopy, and X-ray analysis. A dose- and time-dependent inhibition of proliferation was observed in A549 lung cancer cell after compounds treatment. Hoechst 33258 staining assay and Western blot analysis of LC3-II level showed that the inhibition against A549 cell growth might be attributed to apoptosis and autophagy.Figure optionsDownload full-size imageDownload as PowerPoint slide