Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1371877 | Bioorganic & Medicinal Chemistry Letters | 2009 | 5 Pages |
Abstract
13C7-Labeled iodoacetanilide has been synthesized for specific labeling of sulfhydryl groups of cysteine residues and has been successfully applied to quantitative analysis of peptides and a commercial protein in combination with 13C-unlabeled iodoacetanilide and a MALDI mass spectrometer. Subsequent tandem mass spectrum analysis revealed that 13C7-labeled iodoacetanilide remained intact during the collision-induced dissociation (CID) conditions.
Graphical abstract13C7-Labeled and unlabeled iodoacetanilide that covalently react with cysteine residues have been synthesized and applied to quantitative analysis of peptides and a protein.Figure optionsDownload full-size imageDownload as PowerPoint slide
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Satomi Niwayama, Masoud Zabet-Moghaddam, Sadamu Kurono, Hanjoung Cho,