Affinity-based ranking of ligands for DPP-4 from mixtures

Affinity-based selection strategies have recently emerged as a complement to traditional high throughput screening for the rapid discovery of lead compounds for the large number of protein targets emerging from—omics technologies. Herein, we describe a method for the ranking of mixtures of ligands by affinity selection and apply it to rank order a set of inhibitors for the enzyme dipeptidyl peptidase IV.

Graphical abstractA method for rank ordering ligands by affinity selection is described and demonstrated with a series of inhibitors for DPP-IV.Figure optionsDownload full-size imageDownload as PowerPoint slide