A simple method for monitoring protein–DNA interactions

A simple, efficient and cheap method is reported for monitoring interactions between single stranded desoxyribonucleic acids and proteins, using fluorescence spectroscopy and complexes of 5′-dye–DNA conjugates with bovine serum albumin as probes. In the presence of a single stranded DNA-binding protein the complexes with bovine serum albumin are disrupted, which results in a reduction of fluorescence intensity.

Graphical abstractA simple, efficient and cheap method is reported for monitoring interaction between single stranded desoxyribonucleic acids and proteins by using fluorescence spectroscopy.Figure optionsDownload full-size imageDownload as PowerPoint slide