Synthesis and characterization of a BODIPY-labeled derivative of Soraphen A that binds to acetyl-CoA carboxylase

BODIPY-labeled Soraphen A derivative 4 was synthesized and characterized as an acetyl-CoA carboxylase (ACC) binder. Biophysical measurements indicate that the molecule binds in the biotin carboxylase domain where Soraphen A has been shown to bind. The fluorescent label of the BODIPY can be used to biophysically identify a compound that binds to the Soraphen A site of the biotin carboxylase domain versus the carboxytransferase domain of ACC.

Graphical abstractBODIPY-labeled Soraphen A analog 4 can identify compounds that bind to the Soraphen binding site of the acetyl-CoA carboxylase biotin carboxylase domain.Figure optionsDownload full-size imageDownload as PowerPoint slide