Generating short peptidic ligands for silver nanowires from phage display random libraries

We report the generation of peptide ligands for silver nanowires using a linear 12-mer peptide phage display random library technique. Phage clones that specifically bind the silver nanowires are sequenced after three rounds of biopanning, and obtained DNA sequences suggest that there are a few conserved amino acid residues which may be critical for binding. A selected binding peptide, together with two mutant peptide sequences, were subsequently synthesized on Tentagel resins to examine the importance of both the identities and positions of the conserved amino acid residues.

Graphical abstractWe report the generation of peptide ligands for silver nanowires using a linear 12-mer peptide phage display random library technique. A selected binding peptide, together with two mutant peptide sequences, were subsequently synthesized on Tentagel resins to examine the importance of both the identities and positions of the conserved amino acid residues.Figure optionsDownload full-size imageDownload as PowerPoint slide