| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1374373 | Bioorganic & Medicinal Chemistry Letters | 2006 | 5 Pages |
Six amentoflavone-type biflavonoids, bilobetin (1), ginkgetin (2), 4′,7″-di-O-methyl-amentoflavone (3), 7-O-methyl-isoginkgetin (4), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6), were isolated from the EtOAc fraction of Cephalotaxus koreana Nakai (Cephalotaxaceae) by bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. Among the six biflavonoids isolated, bilobetin (1), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6) significantly increased osteoblast differentiation as assessed by alkaline phosphatase activity, collagen synthesis, and mineralization. Considering structure–activity relationship, methoxyl groups at 4′ and 4‴ in the B rings in amentoflavone-type biflavonoid might be important in osteoblast differentiation. Taken together, our present study suggests therapeutic potential of biflavonoids against bone diseases such as osteoporosis.
Graphical abstractBilobetin (1), sciadopitysin (5), and 7,4′,7″,4‴-O-methyl-amentoflavone (6), biflavonoids isolated from Cephalotaxus koreana increased osteoblast differentiation.Figure optionsDownload full-size imageDownload as PowerPoint slide
