| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1374589 | Bioorganic & Medicinal Chemistry Letters | 2010 | 4 Pages |
Abstract
Here, we developed a reverse transcriptase based method (RTase stop assay) to characterize quadruplex formations in guanine-rich RNAs with high sensitivity and specificity. By using the RTase stop assay, we also revealed a plausible structural polymorphism in biologically important RNAs. The RTase stop assay would provide helpful insight into RNA quadruplex structures and functions, together with other analytical methods, including various footprinting techniques.
Graphical abstractWe developed an enzyme-based method to certify quadruplex formations in biologically important RNAs.Figure optionsDownload full-size imageDownload as PowerPoint slide
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Masaki Hagihara, Keisuke Yoneda, Hiroaki Yabuuchi, Yasushi Okuno, Kazuhiko Nakatani,