Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1374710 | Bioorganic & Medicinal Chemistry Letters | 2006 | 4 Pages |
Abstract
Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose–response for choline (0–50 μM) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (1 μL) and analysis time (<5 s per well).
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Related Topics
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Chemistry
Organic Chemistry
Authors
Maciej Adamczyk, R. Jeffrey Brashear, Phillip G. Mattingly,