Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1374734 | Bioorganic & Medicinal Chemistry Letters | 2006 | 5 Pages |
Abstract
Efficient synthesis of phosphorothioate RNA (PS-RNA) is demonstrated by using phenylacetyl disulfide (PADS) in a mixture of pyridine and acetonitrile (1:1, v/v) for 3 min. Sulfurization is achieved with >99.8% stepwise efficiency. This reagent also performs efficiently during synthesis of RNA containing PS:PO mixed backbone.
Graphical abstractEfficient synthesis of phosphorothioate RNA (PS-RNA) is demonstrated by using phenylacetyl disulfide (PADS) in a mixture of pyridine and acetonitrile (1:1, v/v) for 3 min. Sulfurization is achieved with >99.8% stepwise efficiency. This reagent also performs well during synthesis of RNA containing PS:PO mixed backbone.Figure optionsDownload full-size imageDownload as PowerPoint slide
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Vasulinga T. Ravikumar, Mark Andrade, Recaldo L. Carty, Amy Dan, Steve Barone,