Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1374799 | Bioorganic & Medicinal Chemistry Letters | 2008 | 4 Pages |
Abstract
We developed a method for simply and rapidly detecting cofactors of aptazymes with high sensitivity using unique noncrosslinking gold nanoparticle aggregation. Applying this method to a theophylline-dependent aptazyme, 100 μM, 10 μM, and 1 μM theophylline were detected easily by the naked eye within 10 min, 20 min, and 65 min, respectively. This method is also applicable to other cleavase-aptazymes without altering the probe-DNA sequence on the gold nanoparticle.
Graphical abstractWe easily detected cofactors of aptazymes using noncrosslinking gold nanoparticle aggregation.Figure optionsDownload full-size imageDownload as PowerPoint slide
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Atsushi Ogawa, Mizuo Maeda,