Article ID Journal Published Year Pages File Type
1374799 Bioorganic & Medicinal Chemistry Letters 2008 4 Pages PDF
Abstract

We developed a method for simply and rapidly detecting cofactors of aptazymes with high sensitivity using unique noncrosslinking gold nanoparticle aggregation. Applying this method to a theophylline-dependent aptazyme, 100 μM, 10 μM, and 1 μM theophylline were detected easily by the naked eye within 10 min, 20 min, and 65 min, respectively. This method is also applicable to other cleavase-aptazymes without altering the probe-DNA sequence on the gold nanoparticle.

Graphical abstractWe easily detected cofactors of aptazymes using noncrosslinking gold nanoparticle aggregation.Figure optionsDownload full-size imageDownload as PowerPoint slide

Related Topics
Physical Sciences and Engineering Chemistry Organic Chemistry
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