| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1375030 | Bioorganic & Medicinal Chemistry Letters | 2009 | 4 Pages |
Abstract
Probes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophs They combine the d-threo ketose moiety recognised by transketolase and the side chain of leucine or methionine. These compounds were donor substrates of yeast transketolase leading to the release of the corresponding α-hydroxyaldehydes which could be converted in E. coli by a cascade of reactions into leucine or methionine required for cellular growth.
Graphical abstractProbes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophsFigure optionsDownload full-size imageDownload as PowerPoint slide
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Grégory Simon, Madeleine Bouzon, Franck Charmantray, Virgil Hélaine, Bertrand Légeret, Philippe Marlière, Laurence Hecquet,