Development of a novel fluorescent probe for fluorescence correlation spectroscopic detection of kinase inhibitors

We have developed a fluorescently labeled probe for high-throughput screening of kinase inhibitors using fluorescence correlation spectroscopy. With this probe, we have successfully evaluated the inhibitory activities of known inhibitors of a model kinase, ASK1. Because the probe contains a general kinase inhibitor, staurosporine, we believe that this homogeneous, high-throughput, and simple method can be applied to the inhibitor screening of other kinases as well.

Graphical abstractWith this probe, we successfully evaluated the inhibitory activities of known inhibitors of ASK1.Figure optionsDownload full-size imageDownload as PowerPoint slide