| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1375310 | Bioorganic & Medicinal Chemistry Letters | 2009 | 4 Pages |
In continuation of our studies with stabilized immune modulatory RNA (SIMRA) compounds, we have synthesized novel SIMRA compounds incorporating arabinonucleotides to study their effects on TLR7 and TLR8 activation. The SIMRA compounds containing ara-G, ara-C, ara-U or ara-A substitutions activated TLR8 in HEK293 cells. Interestingly, the SIMRA compound containing ara-C also activated TLR7 and stimulated immune responses in vivo in mice. In human PBMC and pDC assays, SIMRA compounds containing arabinonucleotides induced Th1-type cytokine profiles. These results suggest that SIMRA compounds containing arabinonucleotides act as agonists of TLR7 and TLR8.
Graphical abstractOligoribonucleotides incorporating arabinonucleotides are synthesized and studied as agonists of Toll-like receptors 7 and 8.Figure optionsDownload full-size imageDownload as PowerPoint slide
