Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1375444 | Bioorganic & Medicinal Chemistry Letters | 2005 | 5 Pages |
Random libraries of mRNA 5′-leader sequences were screened to obtain some sequences that can stimulate the translation initiation in a cell-free translation system from wheat embryos as efficiently as the Ω sequence from tobacco mosaic virus. Several sequences that are as useful as the Ω sequence and are homologous to no known sequences survived the screening. We expect that these sequences add useful options to the cell-free protein synthesis system that is becoming a powerful tool in the post-genomic researches.
Graphical abstractTwo novel sequences that enhance translation of downstream open reading frames were obtained by random library selection through isolation of polysomes generated in vitro.Figure optionsDownload full-size imageDownload as PowerPoint slide