| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1375457 | Bioorganic & Medicinal Chemistry Letters | 2005 | 4 Pages |
UDP-GalNAc has been synthesised with high yield from GalNAc, UTP and ATP using recombinant human GalNAc kinase GK2 and UDP-GalNAc pyrophosphorylase AGX1. Both enzymes have been prepared in one step from 1 L cultures of transformed Escherichia coli and the UDP-GalNAc produced has been purified by a simple procedure. The method described is a rapid and efficient means to produce UDP-GalNAc as well as analogues like UDP-N-azidoacetylgalactosamine (UDP-GalNAz).
Graphical abstractUDP-GalNAc has been synthesised with high yield from GalNAc, UTP and ATP using recombinant human GalNAc kinase GK2 and UDP-GalNAc pyrophosphorylase AGX1. The method proved to be applicable to UDP-N-azidoacetylgalactosamine synthesis.Figure optionsDownload full-size imageDownload as PowerPoint slide
