In vitro selection of hemin-binding catalytic RNA

Catalytic RNAs with peroxidase activity were obtained by the in vitro selection of RNA aptamer-binding hemin. One of the RNA aptamers selected showed binding affinity to hemin with a dissociation constant of 0.8 μM and exhibited high peroxidase activity by forming a complex with hemin. The catalytic efficiency of the RNA–hemin complex was 10-fold higher than that of hemin alone.

Graphical abstractIn vitro selection of catalytic RNA with peroxidase activity is reported.Figure optionsDownload full-size imageDownload as PowerPoint slide