Microarray-based label-free detection of RNA using bispyrene-modified 2′-O-methyl oligoribonucleotide as capture and detection probe

A novel oligonucleotide microarray that can detect RNAs without fluorescent labeling of sample RNAs was developed. As a capture and detection probe, bispyrene-modified 2′-O-methyl oligoribonucleotide (OMUpy2), whose fluorescence was dramatically increased when hybridized with its complementary RNA, was adopted. Fluorescence of the OMUpy2 tethered on the glass surface was enhanced as much as 22-fold by the addition of complementary oligoribonucleotide.

Graphical abstractWe developed a novel oligonucleotide microarray for the detection of RNA without fluorescent labeling of target RNA. The technology may contribute to on-site gene diagnostics of various diseases.Figure optionsDownload full-size imageDownload as PowerPoint slide