Interaction of human serum albumin and its clinically relevant modification with oligoribonucleotides

Human serum albumin (HSA) was shown to mediate oligoribonucleotide cleavage. Nonenzymatic glycation of HSA decreased the ribonuclease-like activity of the protein. According to 31P NMR data, both native and glycated albumins induced hydrolysis of RNA molecule through 2′,3′-cyclophosphate intermediates. A feasible mechanism of RNA hydrolysis by native albumin and its clinically relevant modification was discussed.

Graphical abstractRNA hydrolysis in the presence of HSA proceeds via 2′,3′-cyclophosphate intermediates. Nonenzymatic glycation of HSA decreases protein-mediated oligoribonucleotide cleavage with no influence on the cleavage specificity.Figure optionsDownload full-size imageDownload as PowerPoint slide