Synthesis of a coumarin-based europium complex for bioanalyte labeling

A coumarin-based europium chelate ready-to-use for analyte labeling and homogeneous time-resolved fluorescence measurements has been designed. Compound 1 displays three functional elements: an azide reactive spacer arm, a coumarin sensitizer, and a seven-coordinate europium complex. That complex can be excited at 370 nm by inexpensive UV-LEDs as a light excitation source.

Graphical abstractThe synthesis and preliminary evaluation of a labeling compound designed for HTRF measurements with excitation at 370 nm by UV-LEDs is reported.Figure optionsDownload full-size imageDownload as PowerPoint slide