| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1376262 | Bioorganic & Medicinal Chemistry Letters | 2007 | 4 Pages |
Abstract
N-(3-Oxo-acyl) homoserine lactones are used by Gram-negative bacteria to signal the establishment of specific population densities and coordinate population-wide gene expression. Herein we report the antibody-catalyzed hydrolysis of N-(3-oxo-acyl) homoserine lactone (AHL) using a reactive immunization strategy with a squaric monoester monoamide hapten. Kinetic analysis of the most efficient antibody revealed a modest kcat, with AHL hydrolysis competitively inhibited by original squaric monoester monoamide hapten. These studies suggest that antibody catalysis could provide a new avenue for blocking quorum sensing in bacteria.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Sandra De Lamo Marin, Yang Xu, Michael M. Meijler, Kim D. Janda,