New anti-inflammatory ergostane-type ecdysteroids from the sclerotium of Polyporus umbellatus

Bioassay-guided fractionation of the ethyl acetate extract from the sclerotium of Polyporus umbellatus resulted in the isolation of three new ergostane-type ecdysteroids, named polyporoid A (1), B (2), and C (3), together with five known ecdysteroids. The structures of the new compounds were determined on the basis of extensive spectroscopic data (IR, MS, 1H and 13C NMR, and 2D NMR) analyses. All compounds (1–8) exhibited potent anti-inflammatory activity in the test of TPA-induced inflammation (1 μg/ear) in mice, with ID50 values in the range of 0.117–0.682 μM/ear.

Graphical abstractThree new ergostane-type ecdysteroids along with five known ecdysteroids were isolated from the sclerotium of Polyporus umbellatus under bioassay-guided purification. All compounds exhibited potent anti-inflammatory activity in the test of TPA-induced inflammation in mice.Figure optionsDownload full-size imageDownload as PowerPoint slide