| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1376747 | Bioorganic & Medicinal Chemistry Letters | 2006 | 4 Pages |
A fluorescent intercalator displacement assay (G4-FID) has been designed based on the displacement of thiazole orange (TO) positioned onto a quadruplex-forming oligonucleotide by putative ligands. This technique was validated by the use of a set of representative and fully characterized G-quadruplex binders (ranging from pyridodicarboxamide to macrocyclic ligands). To further extend its applicability, a comparative version has been developed which allows a rapid and viable determination of quadruplex- over duplex-selectivity.
Graphical abstractA fluorescent intercalator displacement assay (G4-FID) has been designed based on thiazole orange displacement from a quadruplex-forming oligonucleotide by putative quadruplex-ligands. It allows selection of high-affinity ligands, affinity ranking and viable determination of quadruplex- over duplex-selectivity.Figure optionsDownload full-size imageDownload as PowerPoint slide
