Glycosidase profiling with immobilised glycosidase-inhibiting iminoalditols—A proof-of-concept study

Three typical glycosidase-inhibiting iminoalditols were attached to a polyamine surface displayed on a silicon chip. Exposure to a representative β-glucosidase revealed selective binding events reflecting the different structural features of the inhibitors probed in this study. This provides a proof-of-concept for the successful exploitation of microarrays of typical reversible glycosidase inhibitors of the iminosugar family.

Graphical abstractThe design of an iminoalditol chip and a proof-of-concept study by exposure of the microarray to β-glucosidase from Agrobacterium sp. is reported.Figure optionsDownload full-size imageDownload as PowerPoint slide