| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1377222 | Bioorganic & Medicinal Chemistry Letters | 2006 | 6 Pages |
Abstract
We have used DNA footprinting and fluorescence melting experiments to study the sequence specific binding of an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) to DNA. While the parent compound (thiazotropsin A) binds to the hexanucleotide sequence ACTAGT, changing one of the N-methylpyrrole groups to N-methylimidazole changes the preferred binding sequence to (A/T)CGCG(T/A). Experiments with DNA fragments that contain variants of this sequence suggest that the ligand can also bind, with lower affinity, to sequences which differ from this by 1 bp in any position.
Graphical abstractThiazotropsin B binds to the sequence (A/T)CGCG(T/A).Figure optionsDownload full-size imageDownload as PowerPoint slide
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Andrew J. Hampshire, Hannah Khairallah, Abedawn I. Khalaf, Abdolrasoul H. Ebrahimabadi, Roger D. Waigh, Colin J. Suckling, Tom Brown, Keith R. Fox,