| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1377665 | Bioorganic & Medicinal Chemistry Letters | 2006 | 4 Pages |
The recombinant thioesterase (TE) domain of the picromycin/methymycin synthase (PICS) catalyzes the macrolactonization of 3, the N-acetylcysteamine thioester of seco-10-deoxymethynolide to generate 10-deoxymethynolide (1) with high efficiency. By contrast, 4, the 7-dihydro derivative of seco-thioester 3, undergoes exclusive hydrolysis by PICS TE to seco-acid 5. The recombinant TE domain of 6-deoxyerythronolide B synthase (DEBS TE) shows the same reaction specificity as PICS TE, but with significantly lower activity.
Graphical abstractThe recombinant thioesterase (TE) domain of the picromycin-methymycin synthase (PICS) catalyzes the macrolactonization of 3, the N-acetylcysteamine thioester of seco-10-deoxymethynolide, to generate 10-deoxymethynolide (1) with high efficiency. The recombinant TE domain of 6-deoxyerythronolide B synthase (DEBS TE) shows the same reaction specificity as PICS TE, but with significantly lower activity.Figure optionsDownload full-size imageDownload as PowerPoint slide
