| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1377767 | Bioorganic & Medicinal Chemistry Letters | 2007 | 4 Pages |
Abstract
A significant level of target degradation was caused by bisulfite treatment for methylcytosine-selective hydrolysis. The depyrimidination proceeded via addition of bisulfite to pyrimidines in DNA. The quantification with real-time PCR after conventional bisulfite treatment showed a large decrease in the amount of full-length DNA.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Kazuo Tanaka, Akimitsu Okamoto,