Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1378196 | Bioorganic & Medicinal Chemistry Letters | 2005 | 4 Pages |
Abstract
A sulfur-substituted analogue of trigalacturonic acid (3) was synthesized. The synthesis features the application of 3-cyano-3-(tert-butyldimethylsilyl)oxypropylthioether (CSP) as a novel protective group for thiols. This analogue was designed with the expectation that it would be a stable analogous substrate for endo-polygalacturonase isolated from Stereum purpureum based on computer modeling experiments. Surface plasmon resonance experiments revealed that 3 forms a stable complex with the target enzyme.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Kazunori Yamamoto, Naoki Watanabe, Hiroko Matsuda, Keiichiro Oohara, Tomoyuki Araya, Masaru Hashimoto, Kazuo Miyairi, Isao Okazaki, Minoru Saito, Tetsuya Shimizu, Hiroaki Kato, Toshikatsu Okuno,