Novel fluorescent analogues for transmembrane movement study of polyprenyl phosphates

In order to investigate the transmembrane movement of polyprenyl phosphate across biological membranes, NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled polyprenyl phosphate analogues were prepared. These analogues proved to be possible tools for a direct observation of the transmembrane flip-flop movement of polyprenyl phosphates by use of a sodium dithionite-quenching procedure.

Graphical abstractNovel NBD-labeled fluorescent polyprenyl phosphate analogues were synthesized for transmembrane movement study of polyprenyl phosphates, which may promise a methodology for the direct observation of the lipids flip-flop.Figure optionsDownload full-size imageDownload as PowerPoint slide