Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1378269 | Bioorganic & Medicinal Chemistry Letters | 2007 | 5 Pages |
A topoisomerase I DNA unwinding assay has been used to determine the relative DNA-binding affinities of a model pair of homologous naphthalene diimides. Binding affinity data were corroborated using calorimetric (ITC) and spectrophotometric (titration and Tm) studies, with substituent size playing a significant role in binding. The assay was also used to investigate the mode of binding adopted by several known DNA-binding agents, including SYBR Green and PicoGreen. Some of the compounds exhibited unexpected binding modes.
Graphical abstractTopoisomerase I unwinding assay is used to determine the relative DNA-binding affinities of homologous naphthalene diimides as well as the binding mode adopted by several known DNA-binding agents.Figure optionsDownload full-size imageDownload as PowerPoint slide