Chemico-enzymatic synthesis of a new fluorescent-labeled DNA by PCR with a thymidine nucleotide analogue bearing an acridone derivative

Triphosphate of a thymidine analogue bearing acridone was prepared and incorporated as a substrate for PCR using KOD Dash DNA polymerase forming a new fluorescent-labeled DNA that is useful as a DNA probe.

Graphical abstractTriphosphate of acridone-tagged thymidine analogue was incorporated as a substrate for PCR using KOD Dash DNA polymerase forming a new fluorescent labeled DNA which is useful for a DNA probe.Figure optionsDownload full-size imageDownload as PowerPoint slide