Biomolecular sensor based on fluorescence-labeled aptamer

Fluorescent DNA probes for l-argininamide were developed by a combination of DNA aptamers and fluorophore–quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect l-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities.

Graphical abstractFluorescence-labeled aptamer for l-argininamide was developed by a combination of DNA aptamer and fluorophore–quencher pairs. The fluorescence intensity of the aptamer was reduced to about one-fifth by the addition of l-argininamide. The binding affinities of the modified aptamers were nearly equal to that of its original aptamer.Figure optionsDownload full-size imageDownload as PowerPoint slide