Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1378849 | Bioorganic & Medicinal Chemistry Letters | 2006 | 4 Pages |
A new far-red dual fluorogenic and chromogenic substrate, 5-glycylprolylglycylprolyl-9-di-3-sulfonyl-propylaminobenza[a]phenoxazonium perchlorate (GPGP-2SBPO), was developed for dipeptidyl peptidase IV (DPP-IV) sensing. The glycylprolylglycylprolyl tetrapeptide was chosen as the recognition sequence due to its stability under physiological conditions. In contrast, the truncated substrate, GP-2SBPO, containing only a glycylprolyl peptide, is unstable. Proteolysis of GPGP-2SBPO was assayed by monitoring the absorbance and fluorescence signals from the released fluorochrome, 2SBPO, at 625 and 670 nm, respectively.
Graphical abstractA water-soluble far-red tetrapeptide substrate was developed to sense DPP-IV activity.Figure optionsDownload full-size imageDownload as PowerPoint slide