Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1378906 | Bioorganic & Medicinal Chemistry Letters | 2005 | 5 Pages |
Abstract
One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Aparna Girish, Hongyan Sun, Dawn S.Y. Yeo, Grace Y.J. Chen, Teck-Khiang Chua, Shao Q. Yao,