Aryl O- and S-galactosides and lactosides as specific inhibitors of human galectins-1 and -3: Role of electrostatic potential at O-3

Phase transfer catalyzed reaction was used for the high yielding synthesis of aryl 1-thio-β-d-galacto- and lacto-pyranosides carrying a panel of substituents on the phenyl groups. Best galectin-1 inhibitors were simple p-nitrophenyl thiogalactoside 5a for the monosaccharide and o-nitrophenyl thiolactoside 6f or napthylsulfonyl lactoside 8c, both being 20 times better relative to natural ligands. Relative inhibitory properties as low as 2500 and 40 μM were observed, respectively. The electronic effects of the lactoside aglycons directly influenced the electrostatic potential at O-3, which was associated with the inhibitory potencies against galectin-1.

Graphical abstractAryl O- and S-galactosides, and lactosides were prepared and used for the specific inhibition of galectins-1 and -3. In inhibition of hemagglutination assay against red blood cells, the best inhibitor for galectin-1 was the β-napthylsulfonyl lactoside 8c (40 μM). Electrostatic potential at O-3 correlated with the relative potency.Figure optionsDownload full-size imageDownload as PowerPoint slide