Camptothecin binds to a synthetic peptide identified by a T7 phage display screen

An analysis of non-biotinylated camptothecin (CPT) binding to the C-20-biotinylated CPT binding peptide NSSQSARR was carried out using two methods, quartz-crystal microbalance (QCM) and surface plasmon resonance (SPR). The peptide was immobilized peptide on a sensor chip and showed a dissociation constant (KD) of approximately 0.1 μM against CPT in QCM and SPR experiments.

Graphical abstractAn analysis of non-biotinylated campotothecin (CPT) binding to the C-20-biotinylated CPT binding peptide was carried out using QCM and SPR.Figure optionsDownload full-size imageDownload as PowerPoint slide