| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1379737 | Bioorganic & Medicinal Chemistry Letters | 2005 | 5 Pages |
Abstract
A novel approach for measuring phytase activity is presented. We have developed a new chromophoric substrate analog of phytic acid, 5-O-[6-(benzoylamino)hexyl]-d-myo-inositol-1,2,3,4,6-pentakisphosphate that permits direct measurement of the phosphate ester bond-cleavage reaction using HPLC. This compound, along with its dephosphorylated T-phosphatidylinositol intermediates, are quantified using reversed phase chromatography with UV detection.
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Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Duane F. Berry, David A. Berry,