Detecting diversity: emerging methods to estimate species diversity

•Surveys of species diversity fail to detect species, yielding biased results.•Traditional diversity estimators correct for few causes of detection error.•We examine hierarchical, detection-based models of species diversity.•These can quantify both the number of species detected and the number of species not detected during surveys.•Accounting for imperfect detection can alter conclusions from diversity studies.

Estimates of species richness and diversity are central to community and macroecology and are frequently used in conservation planning. Commonly used diversity metrics account for undetected species primarily by controlling for sampling effort. Yet the probability of detecting an individual can vary among species, observers, survey methods, and sites. We review emerging methods to estimate alpha, beta, gamma, and metacommunity diversity through hierarchical multispecies occupancy models (MSOMs) and multispecies abundance models (MSAMs) that explicitly incorporate observation error in the detection process for species or individuals. We examine advantages, limitations, and assumptions of these detection-based hierarchical models for estimating species diversity. Accounting for imperfect detection using these approaches has influenced conclusions of comparative community studies and creates new opportunities for testing theory.